I toured the biosphere 2 experiment near Tucson Arizona last weekend. I thought it was an impressive structure and an important advancement for understanding.
This experiment sealed people inside to live on a very strict diet - meat once a week - and proved that a closed system could work for long periods. The original experiment is long gone and now the massive building is run by the university of Arizona investigating water return from sloped areas called the LEO project.
I would encourage you to go check it out. Even if you dream of an interstellar colony rather than earth science it is a good approximation for what that would be like. Tour cost was $20 and we came on 2 for 1 tour day. I recommend the tour and Claudio the tour guide is an original player in the original experiment.
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